![]() ![]() Image courtesy of Dr Cimini, Broad Institute. A sample of images featuring specific organelles, extracted from a single Cell Painting assay (bottom right). This protocol provides a powerful method for evaluating the effects of different chemicals on cells, including potential treatments for cancer. Cell painting can be used to identify the phenotypic impact of chemical or genetic perturbations, grouping compounds and/or genes into functional pathways, or identify disease signatures. Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes Mark-Anthony Bray, Shantanu Singh, Han Han, Chadwick T Davis, Blake Borgeson, Cathy. While most published profiling methods use only three dyes, the Cell Painting assay uses six fluorescent stains imaged in five channels. Simple 6-color staining allows researchers to include organelle morphology in analysis, including the nucleus, mitochondria, and actin cytoskeleton. CELLPROFILER CELL PAINTING NAMES SOFTWAREShe is the co-creator of CellProfiler, open-source software for high-throughput biological image analysis, and a co-inventor of the Cell Painting assay, a method for image-based profiling. Researchers from the Broad Institute of MIT and Harvard have published a new protocol in Nature Protocols detailing Cell Painting – a high content screening assay for evaluating about 1500 morphological features of single cells, including shape, texture, size, etc. Carpenter is an American scientist in the field of image analysis for cell biology and artificial intelligence for drug discovery. The term SheleeArt Style was coined by an Australian artist of the same name who created such Dutch Pours. ![]()
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